Antioxidant Activity of Tempeh Ethanolic Extract on Male Swiss Mouse Brain

Tempeh is an Indonesian food made of soybean (Glycine max (L.) Merr.) by fermentation using Rhizopus spp mold. Soybean has a high content protein, fatty acids, vitamins, and isoflavone glycosides. Isoflavone glycosides are poorly absorbed in the human intestine. Fermentation hydrolyzes into aglycones which easily absorbed. three main isoflavones, they genistein, daidzein, glycitein. They good antioxidants have estrogen-like activity. This research studies antioxidant activity tempeh ethanolic extract determining concentration superoxide dismutase (SOD) glutathione peroxidase (GPx) brain tissue male Swiss mice. All test substances were diluted 0.5% CMC-Na solution administered orally once daily for 12 days. The 0.5 mL 15% ethanol (16.67 mL/kg BW) was 1 hour before other substances. (500 mg/kg as treatment. Donepezil-HCl (1 genistein (2.55 used positive control groups. mouse SOD GPx determined on 13th day. Results showed significant depletion administration. Compared to group, mice with no change but little decrease SOD. group significantly higher than donepezil-HCl genistein. Briefly concluded that better